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Guilford, William

William Guilford

Primary Appointment

Biomedical Engineering

Contact Information

PO Box 800759
Charlottesville, Virginia 22908
Telephone: 434-243-2740

Research Interests

Molecular Mechanics of Muscle Contraction and Cell Movement

Research Description

Our goal is to understand the molecular mechanisms by which cells move, with particular emphasis on muscle contraction. We examine the mechanics of these processes at the level of individual molecules using laser traps, fluorescence microscopy, and reconstituted motile and adhesive systems. These allow us to better define the molecular underpinnings of many cell movements, and the molecular basis of many diseases.

Selected Publications

Clobes AM, Guilford WH, Loop 2 of myosin is a force-dependent inhibitor of the rigor bond., 2014; Journal of muscle research and cell motility. 35(2) 143-52 PMID: 24500136 | PMCID: PMC4096411

Judge JM, Stukenborg GJ, Johnston WF, Guilford WH, Slingluff CL, Hallowell PT, Design, Development, and Evaluation of a Novel Retraction Device for Gallbladder Extraction During Laparoscopic Cholecystectomy., 2013; Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. () PMID: 23897085 |

Guilford WH, Bloodgood RA, Laser trap measurements of flagellar membrane motility., 2013; Methods in enzymology. 525() 85-107 PMID: 23522466 |

Reed ZH, Guilford W, Park JW, Thermal denaturation of tilapia myosin and its subunits as affected by constantly increasing temperature., 2012; Journal of food science. 76(7) C1018-24 PMID: 22417538 |

Rao VS, Clobes AM, Guilford WH, Force spectroscopy reveals multiple "closed states" of the muscle thin filament., 2011; The Journal of biological chemistry. 286(27) 24135-41 PMID: 21597115 | PMCID: PMC3129194

Evangelista AM, Rao VS, Filo AR, Marozkina NV, Doctor A, Jones DR, Gaston B, Guilford WH, Direct regulation of striated muscle myosins by nitric oxide and endogenous nitrosothiols., 2010; PloS one. 5(6) e11209 PMID: 20585450 | PMCID: PMC2887846

Python JL, Wilson KO, Snook JH, Guo B, Guilford WH, The viscoelastic properties of microvilli are dependent upon the cell-surface molecule., 2010; Biochemical and biophysical research communications. 397(3) 621-5 PMID: 20570653 | PMCID: PMC2907673

Snook JH, Guilford WH, The Effects of Load on E-Selectin Bond Rupture and Bond Formation., 2010; Cellular and molecular bioengineering. 3(2) 128-138 PMID: 20526425 | PMCID: PMC2880517

Laib JA, Marin JA, Bloodgood RA, Guilford WH, The reciprocal coordination and mechanics of molecular motors in living cells., 2009; Proceedings of the National Academy of Sciences of the United States of America. 106(9) 3190-5 PMID: 19221028 | PMCID: PMC2651315

Guilford WH, Experimental case studies to engage higher cognitive skills., 2009; Advances in physiology education. 33(4) 358-9 PMID: 19948689 | PMCID: PMC3776424

Rao VS, Marongelli EN, Guilford WH, Phosphorylation of tropomyosin extends cooperative binding of myosin beyond a single regulatory unit., 2008; Cell motility and the cytoskeleton. 66(1) 10-23 PMID: 18985725 | PMCID: PMC2770177

Guilford WH, Downs KE, Royce TJ, Knowledge of prenatal health care among Costa Rican and Panamanian women., 2008; Revista panamericana de salud p?blica = Pan American journal of public health. 23(6) 369-76 PMID: 18644204 |

Paschall CD, Guilford WH, Lawrence MB, Enhancement of L-selectin, but not P-selectin, bond formation frequency by convective flow., 2007; Biophysical journal. 94(3) 1034-45 PMID: 17890384 | PMCID: PMC2186251

Rao VS, La Bonte LR, Xu Y, Yang Z, French BA, Guilford WH, Alterations to myofibrillar protein function in nonischemic regions of the heart early after myocardial infarction., 2007; American journal of physiology. Heart and circulatory physiology. 293(1) H654-9 PMID: 17400716 |

Snook JH, Li J, Helmke BP, Guilford WH, Peroxynitrite inhibits myofibrillar protein function in an in vitro assay of motility., 2007; Free radical biology & medicine. 44(1) 14-23 PMID: 18045543 | PMCID: PMC2180163

Guo B, Guilford WH, Mechanics of actomyosin bonds in different nucleotide states are tuned to muscle contraction., 2006; Proceedings of the National Academy of Sciences of the United States of America. 103(26) 9844-9 PMID: 16785439 | PMCID: PMC1502541

Guilford WH, "Shrink wrapping" lectures: teaching cell and molecular biology within the context of human pathologies., 2005; Cell biology education. 4(2) 138-42 PMID: 15917872 | PMCID: PMC1103715

Guilford WH, Tournas JA, Dascalu D, Watson DS, Creating multiple time-shared laser traps with simultaneous displacement detection using digital signal processing hardware., 2004; Analytical biochemistry. 326(2) 153-66 PMID: 15003556 |

Guo B, Guilford WH, The tail of myosin reduces actin filament velocity in the in vitro motility assay., 2004; Cell motility and the cytoskeleton. 59(4) 264-72 PMID: 15505809 |

Rinko LJ, Lawrence MB, Guilford WH, The molecular mechanics of P- and L-selectin lectin domains binding to PSGL-1., 2003; Biophysical journal. 86(1) 544-54 PMID: 14695299 | PMCID: PMC1303823

Guilford WH, Teaching peer review and the process of scientific writing., 2002; Advances in physiology education. 25(1) 167-75 PMID: 11824193 |

Cheezum MK, Walker WF, Guilford WH, Quantitative comparison of algorithms for tracking single fluorescent particles., 2001; Biophysical journal. 81(4) 2378-88 PMID: 11566807 | PMCID: PMC1301708

Guilford WH, Warshaw DM, The molecular mechanics of smooth muscle myosin., 1998; Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 119(3) 451-8 PMID: 9734329 |

Guilford WH, Dupuis DE, Kennedy G, Wu J, Patlak JB, Warshaw DM, Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap., 1997; Biophysical journal. 72(3) 1006-21 PMID: 9138552 | PMCID: PMC1184489

Dupuis DE, Guilford WH, Wu J, Warshaw DM, Actin filament mechanics in the laser trap., 1997; Journal of muscle research and cell motility. 18(1) 17-30 PMID: 9147990 |