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Peirce-Cottler, Shayn

Shayn Peirce-Cottler

Primary Appointment

Biomedical Engineering

Contact Information

PO Box 800759
Charlottesville, VA 22908
Telephone: 434-243-5795
Email: smp6p@virginia.edu
Website: http://www.bme.virginia.edu/peirce/

Research Interests

Tissue Engineering and Regeneration, Computational Systems Biology, Vascular Growth and Remodeling, Stem Cell Therapies

Research Description

The Important Role of the Microcirculation: Every organ in the body is dependent on blood flow to provide the necessary oxygen and nutrients in order to stay alive. The circulatory system is responsible for delivering blood to and from all of the tissues, and the microcirculation is the set of the smallest blood vessels in the body. (Microvessels are less than 100 micrometers in diameter, and they can only be visualized using a microscope!) Our research is interested in understanding how microvessels grow and remodel during normal physiological development and in the setting of different important diseases where their involvement in disease progression is absolutely central, such as heart disease, peripheral vascular disease, diabetic retinopathy, cancer, and chronic wound formation. The Microcirculation in Tissue Engineering and Regenerative Medicine: We are are also interested in applying our knowledge of the microcirculation in order to grow new tissues (tissue engineering) and regenerate damaged tissues in the body (tissue regeneration). In fact, without a blood supply (ie. without microvessels) tissues beyond the small size of 1 cubic millimeter cannot survive in the body. Therefore, our research aims to address a critical bottleneck for all of tissue engineering and regenerative medicine aspirations: growing new functional and sustainable microvessels that can deliver blood to the tissues that we are trying to heal and/or replace. Specific Research Goals: The overarching goals of our research are to: 1) understand how tissues, or collections of biological cells and their extracellular matrix environment, grow and adapt in response to physiological and pathological environmental (i.e. biochemical and mechanical) stimuli, and 2) use this information to develop therapeutic strategies for invoking/promoting tissue regeneration and repair. We are predominantly interested in pursuing these goals within the context of the adult microvascular system, which is essential in many human diseases, including heart disease, cancer, and chronic wounds. All of our projects combine multi-cell computational modeling with experimental analyses.

Selected Publications

Virgilio KM, Martin KS, Peirce SM, Blemker SS, Multiscale models of skeletal muscle reveal the complex effects of muscular dystrophy on tissue mechanics and damage susceptibility., 2015; Interface focus. 5(2) 20140080 PMID: 25844152 | PMCID: PMC4342948

Heuslein JL, Li X, Murrell KP, Annex BH, Peirce SM, Price RJ, Computational Network Model Prediction of Hemodynamic Alterations Due to Arteriolar Rarefaction and Estimation of Skeletal Muscle Perfusion in Peripheral Arterial Disease., 2015; Microcirculation (New York, N.Y. : 1994). 22(5) 360-9 PMID: 25866235 | PMCID: PMC4506703

Murfee WL, Sweat RS, Tsubota K, Mac Gabhann F, Khismatullin D, Peirce SM, Applications of computational models to better understand microvascular remodelling: a focus on biomechanical integration across scales., 2015; Interface focus. 5(2) 20140077 PMID: 25844149 | PMCID: PMC4342945

Cronk SM, Kelly-Goss MR, Ray HC, Mendel TA, Hoehn KL, Bruce AC, Dey BK, Guendel AM, Tavakol DN, Herman IM, Peirce SM, Yates PA, Adipose-derived stem cells from diabetic mice show impaired vascular stabilization in a murine model of diabetic retinopathy., 2015; Stem cells translational medicine. 4(5) 459-67 PMID: 25769654 | PMCID: PMC4414213

Martin KS, Blemker SS, Peirce SM, Agent-based computational model investigates muscle-specific responses to disuse-induced atrophy., 2015; Journal of applied physiology (Bethesda, Md. : 1985). 118(10) 1299-309 PMID: 25722379 | PMCID: PMC4436981

Seaman SA, Tannan SC, Cao Y, Peirce SM, Lin KY, Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., 2015; Plastic and reconstructive surgery. 136(2) 189e-99e PMID: 26218393 |

Walpole J, Chappell JC, Cluceru JG, Mac Gabhann F, Bautch VL, Peirce SM, Agent-based model of angiogenesis simulates capillary sprout initiation in multicellular networks., 2015; Integrative biology : quantitative biosciences from nano to macro. () PMID: 26158406 |

Agrawal H, Shang H, Sattah AP, Yang N, Peirce SM, Katz AJ, Human adipose-derived stromal/stem cells demonstrate short-lived persistence after implantation in both an immunocompetent and an immunocompromised murine model., 2014; Stem cell research & therapy. 5(6) 142 PMID: 25523792 | PMCID: PMC4445497

Bruce AC, Kelly-Goss MR, Heuslein JL, Meisner JK, Price RJ, Peirce SM, Monocytes are recruited from venules during arteriogenesis in the murine spinotrapezius ligation model., 2014; Arteriosclerosis, thrombosis, and vascular biology. 34(9) 2012-22 PMID: 24969773 | PMCID: PMC4373588

Okutsu M, Call JA, Lira VA, Zhang M, Donet JA, French BA, Martin KS, Peirce-Cottler SM, Rembold CM, Annex BH, Yan Z, Extracellular superoxide dismutase ameliorates skeletal muscle abnormalities, cachexia, and exercise intolerance in mice with congestive heart failure., 2014; Circulation. Heart failure. 7(3) 519-30 PMID: 24523418 | PMCID: PMC4080303

Kelly-Goss MR, Sweat RS, Stapor PC, Peirce SM, Murfee WL, Targeting pericytes for angiogenic therapies., 2013; Microcirculation (New York, N.Y. : 1994). 21(4) 345-57 PMID: 24267154 | PMCID: PMC4079092

Walpole J, Papin JA, Peirce SM, Multiscale computational models of complex biological systems., 2013; Annual review of biomedical engineering. 15() 137-54 PMID: 23642247 | PMCID: PMC3970111

Guendel AM, Martin KS, Cutts J, Foley PL, Bailey AM, Mac Gabhann F, Cardinal TR, Peirce SM, Murine spinotrapezius model to assess the impact of arteriolar ligation on microvascular function and remodeling., 2013; Journal of visualized experiments : JoVE. (73) e50218 PMID: 23486360 | PMCID: PMC3622090

Mendel TA, Clabough EB, Kao DS, Demidova-Rice TN, Durham JT, Zotter BC, Seaman SA, Cronk SM, Rakoczy EP, Katz AJ, Herman IM, Peirce SM, Yates PA, Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy., 2013; PloS one. 8(5) e65691 PMID: 23741506 | PMCID: PMC3669216

Awojoodu AO, Ogle ME, Sefcik LS, Bowers DT, Martin K, Brayman KL, Lynch KR, Peirce-Cottler SM, Botchwey E, Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis., 2013; Proceedings of the National Academy of Sciences of the United States of America. 110(34) 13785-90 PMID: 23918395 | PMCID: PMC3752259

Taylor AC, Mendel TA, Mason KE, Degen KE, Yates PA, Peirce SM, Attenuation of ephrinB2 reverse signaling decreases vascularized area and preretinal vascular tuft formation in the murine model of oxygen-induced retinopathy., 2012; Investigative ophthalmology & visual science. 53(9) 5462-70 PMID: 22789927 | PMCID: PMC3948501

Yang M, Stapor PC, Peirce SM, Betancourt AM, Murfee WL, Rat mesentery exteriorization: a model for investigating the cellular dynamics involved in angiogenesis., 2012; Journal of visualized experiments : JoVE. (63) e3954 PMID: 22643964 | PMCID: PMC3466932

Peirce SM, Mac Gabhann F, Bautch VL, Integration of experimental and computational approaches to sprouting angiogenesis., 2012; Current opinion in hematology. 19(3) 184-91 PMID: 22406822 | PMCID: PMC4132663

Billaud M, Ross JA, Greyson MA, Bruce AC, Seaman SA, Heberlein KR, Han J, Best AK, Peirce SM, Isakson BE, A new method for in vivo visualization of vessel remodeling using a near-infrared dye., 2011; Microcirculation (New York, N.Y. : 1994). 18(3) 163-71 PMID: 21418375 | PMCID: PMC3081403

Morris E, Kesser BW, Peirce-Cottler S, Keeley M, Development and validation of a novel ear simulator to teach pneumatic otoscopy., 2011; Simulation in healthcare : journal of the Society for Simulation in Healthcare. 7(1) 22-6 PMID: 21937958 |

Hayenga HN, Thorne BC, Peirce SM, Humphrey JD, Ensuring congruency in multiscale modeling: towards linking agent based and continuum biomechanical models of arterial adaptation., 2011; Annals of biomedical engineering. 39(11) 2669-82 PMID: 21809144 | PMCID: PMC3207323

Hashambhoy YL, Chappell JC, Peirce SM, Bautch VL, Mac Gabhann F, Computational modeling of interacting VEGF and soluble VEGF receptor concentration gradients., 2011; Frontiers in physiology. 2() 62 PMID: 22007175 | PMCID: PMC3185289

Seaman ME, Peirce SM, Kelly K, Rapid analysis of vessel elements (RAVE): a tool for studying physiologic, pathologic and tumor angiogenesis., 2011; PloS one. 6(6) e20807 PMID: 21694777 | PMCID: PMC3111429

Bruce AC, Peirce SM, Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model., 2011; Microcirculation (New York, N.Y. : 1994). 19(2) 143-54 PMID: 21954923 | PMCID: PMC3262124

Amos PJ, Mulvey CL, Seaman SA, Walpole J, Degen KE, Shang H, Katz AJ, Peirce SM, Hypoxic culture and in vivo inflammatory environments affect the assumption of pericyte characteristics by human adipose and bone marrow progenitor cells., 2011; American journal of physiology. Cell physiology. 301(6) C1378-88 PMID: 21865587 | PMCID: PMC3233793

Thorne BC, Hayenga HN, Humphrey JD, Peirce SM, Toward a multi-scale computational model of arterial adaptation in hypertension: verification of a multi-cell agent based model., 2011; Frontiers in physiology. 2() 20 PMID: 21720536 | PMCID: PMC3118494

Sefcik LS, Aronin CE, Awojoodu AO, Shin SJ, Mac Gabhann F, MacDonald TL, Wamhoff BR, Lynch KR, Peirce SM, Botchwey EA, Selective activation of sphingosine 1-phosphate receptors 1 and 3 promotes local microvascular network growth., 2010; Tissue engineering. Part A. 17(5) 617-29 PMID: 20874260 | PMCID: PMC3043977

Mac Gabhann F, Peirce SM, Collateral capillary arterialization following arteriolar ligation in murine skeletal muscle., 2010; Microcirculation (New York, N.Y. : 1994). 17(5) 333-47 PMID: 20618691 | PMCID: PMC2907254

Taylor AC, Seltz LM, Yates PA, Peirce SM, Chronic whole-body hypoxia induces intussusceptive angiogenesis and microvascular remodeling in the mouse retina., 2010; Microvascular research. 79(2) 93-101 PMID: 20080108 | PMCID: PMC2828864

Benedict KF, Mac Gabhann F, Amanfu RK, Chavali AK, Gianchandani EP, Glaw LS, Oberhardt MA, Thorne BC, Yang JH, Papin JA, Peirce SM, Saucerman JJ, Skalak TC, Systems analysis of small signaling modules relevant to eight human diseases., 2010; Annals of biomedical engineering. 39(2) 621-35 PMID: 21132372 | PMCID: PMC3033523

Glaw JT, Skalak TC, Peirce SM, Inhibition of canonical Wnt signaling increases microvascular hemorrhaging and venular remodeling in adult rats., 2010; Microcirculation (New York, N.Y. : 1994). 17(5) 348-57 PMID: 20618692 | PMCID: PMC2904644

Petrie Aronin CE, Sefcik LS, Tholpady SS, Tholpady A, Sadik KW, Macdonald TL, Peirce SM, Wamhoff BR, Lynch KR, Ogle RC, Botchwey EA, FTY720 promotes local microvascular network formation and regeneration of cranial bone defects., 2009; Tissue engineering. Part A. 16(6) 1801-9 PMID: 20038198 | PMCID: PMC2949231

Volsky PG, Hughley BB, Peirce SM, Kesser BW, Construct validity of a simulator for myringotomy with ventilation tube insertion., 2009; Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery. 141(5) 603-608.e1 PMID: 19861198 |

Amos PJ, Kapur SK, Stapor PC, Shang H, Bekiranov S, Khurgel M, Rodeheaver GT, Peirce SM, Katz AJ, Human adipose-derived stromal cells accelerate diabetic wound healing: impact of cell formulation and delivery., 2009; Tissue engineering. Part A. 16(5) 1595-606 PMID: 20038211 | PMCID: PMC2952117