Mobile Menu Button

Gelfand, Bradley

Bradley Gelfand

Primary Appointment

Ophthalmology

Contact Information

Email: bdg3y@virginia.edu
Website: http://gelfandlab.weebly.com

Research Interests

Blinding disease age-related macular degeneration, utilizing the tools of immunology, molecular biology, and engineering.

Research Description

One major focus of our work is to study the cellular and molecular consequences of the blood supply in the eye. Specifically, we are interested in quantifying the biomechanical changes that occur in the choroidal vasculature in healthy and diseased eyes, and how these micro-environmental cues affect the biology and pathology of the eye. To accomplish this, we perform high-resolution 3-D microscopy to generate accurate maps of the human choroidal anatomy in health and in disease which is utilized for computational fluid dynamic modeling. To study the influence of choroidal hemodynamics on ocular health, we employ a sophisticated cell culture system which is capable of reproducing the hemodynamic environment of the human eye.

Selected Publications

Kerur N, Fukuda S, Banerjee D, Kim Y, Fu D, Apicella I, Varshney A, Yasuma R, Fowler BJ, Baghdasaryan E, Marion KM, Huang X, Yasuma T, Hirano Y, Serbulea V, Ambati M, Ambati VL, Kajiwara Y, Ambati K, Hirahara S, Bastos-Carvalho A, Ogura Y, Terasak..., cGAS drives noncanonical-inflammasome activation in age-related macular degeneration., 2017; Nature medicine. () PMID: 29176737 | PMCID: PMC5760363

Gelfand BD, Ambati J, A Revised Hemodynamic Theory of Age-Related Macular Degeneration., 2016; Trends in molecular medicine. 22(8) 656-70 PMID: 27423265 | PMCID: PMC4969140

Bogdanovich S, Kim Y, Mizutani T, Yasuma R, Tudisco L, Cicatiello V, Bastos-Carvalho A, Kerur N, Hirano Y, Baffi JZ, Tarallo V, Li S, Yasuma T, Arpitha P, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ruvo M, Sandomenico A, Nozaki M, Ijim..., Human IgG1 antibodies suppress angiogenesis in a target-independent manner., 2016; Signal transduction and targeted therapy. 1() PMID: 26918197 | PMCID: PMC4763941

Yasuma R, Cicatiello V, Mizutani T, Tudisco L, Kim Y, Tarallo V, Bogdanovich S, Hirano Y, Kerur N, Li S, Yasuma T, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ambati BK, Helmers SB, Lundberg IE, Viklicky O, Leusen JH, Verbeek JS, Gelfan..., Intravenous immune globulin suppresses angiogenesis in mice and humans., 2016; Signal transduction and targeted therapy. 1() PMID: 26925256 | PMCID: PMC4768485

Mizutani T, Fowler BJ, Kim Y, Yasuma R, Krueger LA, Gelfand BD, Ambati J, Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice., 2015; Investigative ophthalmology & visual science. 56(12) 7122-9 PMID: 26529046 | PMCID: PMC4634627

Gelfand BD, Wright CB, Kim Y, Yasuma T, Yasuma R, Li S, Fowler BJ, Bastos-Carvalho A, Kerur N, Uittenbogaard A, Han YS, Lou D, Kleinman ME, McDonald WH, N??ez G, Georgel P, Dunaief JL, Ambati J, Iron Toxicity in the Retina Requires Alu RNA and the NLRP3 Inflammasome., 2015; Cell reports. 11(11) 1686-93 PMID: 26074074 | PMCID: PMC4481133

Fowler BJ, Gelfand BD, Kim Y, Kerur N, Tarallo V, Hirano Y, Amarnath S, Fowler DH, Radwan M, Young MT, Pittman K, Kubes P, Agarwal HK, Parang K, Hinton DR, Bastos-Carvalho A, Li S, Yasuma T, Mizutani T, Yasuma R, Wright C, Ambati J, Nucleoside reverse transcriptase inhibitors possess intrinsic anti-inflammatory activity., 2014; Science (New York, N.Y.). 346(6212) 1000-3 PMID: 25414314 | PMCID: PMC4274127

Kim Y, Tarallo V, Kerur N, Yasuma T, Gelfand BD, Bastos-Carvalho A, Hirano Y, Yasuma R, Mizutani T, Fowler BJ, Li S, Kaneko H, Bogdanovich S, Ambati BK, Hinton DR, Hauswirth WW, Hakem R, Wright C, Ambati J, DICER1/Alu RNA dysmetabolism induces Caspase-8-mediated cell death in age-related macular degeneration., 2014; Proceedings of the National Academy of Sciences of the United States of America. 111(45) 16082-7 PMID: 25349431 | PMCID: PMC4234570

Hirano Y, Yasuma T, Mizutani T, Fowler BJ, Tarallo V, Yasuma R, Kim Y, Bastos-Carvalho A, Kerur N, Gelfand BD, Bogdanovich S, He S, Zhang X, Nozaki M, Ijima R, Kaneko H, Ogura Y, Terasaki H, Nagai H, Haro I, N??ez G, Ambati BK, Hinton DR, Ambati J, IL-18 is not therapeutic for neovascular age-related macular degeneration., 2014; Nature medicine. 20(12) 1372-5 PMID: 25473914 | PMCID: PMC4275546

Ambati J, Atkinson JP, Gelfand BD, Immunology of age-related macular degeneration., 2013; Nature reviews. Immunology. 13(6) 438-51 PMID: 23702979 | PMCID: PMC3941009

Kerur N, Hirano Y, Tarallo V, Fowler BJ, Bastos-Carvalho A, Yasuma T, Yasuma R, Kim Y, Hinton DR, Kirschning CJ, Gelfand BD, Ambati J, TLR-independent and P2X7-dependent signaling mediate Alu RNA-induced NLRP3 inflammasome activation in geographic atrophy., 2013; Investigative ophthalmology & visual science. 54(12) 7395-401 PMID: 24114535 | PMCID: PMC3825570

Feaver RE, Gelfand BD, Blackman BR, Human haemodynamic frequency harmonics regulate the inflammatory phenotype of vascular endothelial cells., 2013; Nature communications. 4() 1525 PMID: 23443553 | PMCID: PMC4100071

Dridi S, Hirano Y, Tarallo V, Kim Y, Fowler BJ, Ambati BK, Bogdanovich S, Chiodo VA, Hauswirth WW, Kugel JF, Goodrich JA, Ponicsan SL, Hinton DR, Kleinman ME, Baffi JZ, Gelfand BD, Ambati J, ERK1/2 activation is a therapeutic target in age-related macular degeneration., 2012; Proceedings of the National Academy of Sciences of the United States of America. 109(34) 13781-6 PMID: 22869729 | PMCID: PMC3427082

Tarallo V, Hirano Y, Gelfand BD, Dridi S, Kerur N, Kim Y, Cho WG, Kaneko H, Fowler BJ, Bogdanovich S, Albuquerque RJ, Hauswirth WW, Chiodo VA, Kugel JF, Goodrich JA, Ponicsan SL, Chaudhuri G, Murphy MP, Dunaief JL, Ambati BK, Ogura Y, Yoo JW, Lee ..., DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88., 2012; Cell. 149(4) 847-59 PMID: 22541070 | PMCID: PMC3351582

Kleinman ME, Kaneko H, Cho WG, Dridi S, Fowler BJ, Blandford AD, Albuquerque RJ, Hirano Y, Terasaki H, Kondo M, Fujita T, Ambati BK, Tarallo V, Gelfand BD, Bogdanovich S, Baffi JZ, Ambati J, Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3., 2011; Molecular therapy : the journal of the American Society of Gene Therapy. 20(1) 101-8 PMID: 21988875 | PMCID: PMC3255577

Kaneko H, Dridi S, Tarallo V, Gelfand BD, Fowler BJ, Cho WG, Kleinman ME, Ponicsan SL, Hauswirth WW, Chiodo VA, Karik? K, Yoo JW, Lee DK, Hadziahmetovic M, Song Y, Misra S, Chaudhuri G, Buaas FW, Braun RE, Hinton DR, Zhang Q, Grossniklaus HE, Prov..., DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration., 2011; Nature. 471(7338) 325-30 PMID: 21297615 | PMCID: PMC3077055

Gelfand BD, Meller J, Pryor AW, Kahn M, Bortz PD, Wamhoff BR, Blackman BR, Hemodynamic activation of beta-catenin and T-cell-specific transcription factor signaling in vascular endothelium regulates fibronectin expression., 2011; Arteriosclerosis, thrombosis, and vascular biology. 31(7) 1625-33 PMID: 21527747 | PMCID: PMC3134525

Feaver RE, Gelfand BD, Wang C, Schwartz MA, Blackman BR, Atheroprone hemodynamics regulate fibronectin deposition to create positive feedback that sustains endothelial inflammation., 2010; Circulation research. 106(11) 1703-11 PMID: 20378855 | PMCID: PMC2891748

Harry BL, Sanders JM, Feaver RE, Lansey M, Deem TL, Zarbock A, Bruce AC, Pryor AW, Gelfand BD, Blackman BR, Schwartz MA, Ley K, Endothelial cell PECAM-1 promotes atherosclerotic lesions in areas of disturbed flow in ApoE-deficient mice., 2008; Arteriosclerosis, thrombosis, and vascular biology. 28(11) 2003-8 PMID: 18688018 | PMCID: PMC2651147

Gelfand BD, Epstein FH, Blackman BR, Spatial and spectral heterogeneity of time-varying shear stress profiles in the carotid bifurcation by phase-contrast MRI., 2006; Journal of magnetic resonance imaging : JMRI. 24(6) 1386-92 PMID: 17083089 |