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Kenworthy, Anne

Anne Kenworthy

Primary Appointment

Molecular Physiology and Biological Physics

Contact Information

Telephone: (434) 982-3256
Email: akk7hp@virginia.edu

Research Interests

Architecture and function of biological membranes

Research Description

Research Description One of the major goals of my research program is to understand the role of membrane microdomains such as lipid rafts and caveolae in health and disease. We address this question using a combination of live cell imaging, cell biology, biophysics, structural, and biochemical approaches. Current projects ongoing in the lab include: 1. How do proteins and lipid cooperate to build functional raft domains? 2. How do bacterial toxins exploit lipid rafts as a mechanism to enter host cells? 3. How do cholesterol and lipid rafts contribute to the progression of Alzheimer's disease? 4. How do mutations in caveolin-1 and defects in caveolae cause human diseases such as breast cancer and pulmonary arterial hypertension? 5. What is the structural basis for caveolae formation?

Selected Publications

Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport., 2019; Nature communications. 10(1) 428 PMID: 30683896 | PMCID: PMC6347608

Foegeding NJ, Raghunathan K, Campbell AM, Kim SW, Lau KS, Kenworthy AK, Cover TL, Ohi MD, Intracellular Degradation of Helicobacter pylori VacA Toxin as a Determinant of Gastric Epithelial Cell Viability., 2019; Infection and immunity. 87(4) PMID: 30692181 | PMCID: PMC6434121

Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, Publisher Correction: Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport., 2019; Nature communications. 10(1) 919 PMID: 30783093 | PMCID: PMC6381088

Raghunathan K, Kenworthy AK, Dynamic pattern generation in cell membranes: Current insights into membrane organization., 2018; Biochimica et biophysica acta. Biomembranes. () PMID: 29752898 |

Raghunathan K, Foegeding NJ, Campbell AM, Cover TL, Ohi MD, Kenworthy AK, Determinants of Raft Partitioning of the Helicobacter pylori Pore-Forming Toxin VacA., 2018; Infection and immunity. 86(5) PMID: 29531133 | PMCID: PMC5913846

Kenworthy AK, Bigger Isn't Always Better: Bulking Up Impedes Receptor Internalization., 2018; Biophysical journal. 114(6) 1255-1256 PMID: 29590581 | PMCID: PMC5883612

Saito-Diaz K, Benchabane H, Tiwari A, Tian A, Li B, Thompson JJ, Hyde AS, Sawyer LM, Jodoin JN, Santos E, Lee LA, Coffey RJ, Beauchamp RD, Williams CS, Kenworthy AK, Robbins DJ, Ahmed Y, Lee E, APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway., 2018; Developmental cell. 44(5) 566-581.e8 PMID: 29533772 | PMCID: PMC5884143

Copeland CA, Han B, Tiwari A, Austin ED, Loyd JE, West JD, Kenworthy AK, A disease-associated frameshift mutation in caveolin-1 disrupts caveolae formation and function through introduction of a de novo ER retention signal., 2017; Molecular biology of the cell. 28(22) 3095-3111 PMID: 28904206 | PMCID: PMC5662265

Simunovic M, Manneville JB, Renard HF, Evergren E, Raghunathan K, Bhatia D, Kenworthy AK, Voth GA, Prost J, McMahon HT, Johannes L, Bassereau P, Callan-Jones A, Friction Mediates Scission of Tubular Membranes Scaffolded by BAR Proteins., 2017; Cell. 170(1) 172-184.e11 PMID: 28648660 | PMCID: PMC5576516

Tiwari A, Copeland CA, Han B, Hanson CA, Raghunathan K, Kenworthy AK, Caveolin-1 is an aggresome-inducing protein., 2016; Scientific reports. 6() 38681 PMID: 27929047 | PMCID: PMC5144149

Han B, Copeland CA, Kawano Y, Rosenzweig EB, Austin ED, Shahmirzadi L, Tang S, Raghunathan K, Chung WK, Kenworthy AK, Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy., 2016; Traffic (Copenhagen, Denmark). 17(12) 1297-1312 PMID: 27717241 | PMCID: PMC5197452

Klaus CJ, Raghunathan K, DiBenedetto E, Kenworthy AK, Analysis of diffusion in curved surfaces and its application to tubular membranes., 2016; Molecular biology of the cell. 27(24) 3937-3946 PMID: 27733625 | PMCID: PMC5170615

Cui Y, Kenworthy AK, Edidin M, Divan R, Rosenmann D, Wang P, Analyzing Single Giant Unilamellar Vesicles With a Slotline-Based RF Nanometer Sensor., 2016; IEEE transactions on microwave theory and techniques. 64(4) 1339-1347 PMID: 27713585 | PMCID: PMC5046228

Han B, Copeland CA, Tiwari A, Kenworthy AK, Assembly and Turnover of Caveolae: What Do We Really Know?, 2016; Frontiers in cell and developmental biology. 4() 68 PMID: 27446919 | PMCID: PMC4921483

Kraft LJ, Dowler J, Manral P, Kenworthy AK, Size, organization, and dynamics of soluble SQSTM1 and LC3-SQSTM1 complexes in living cells., 2016; Autophagy. 12(9) 1660-74 PMID: 27442348 | PMCID: PMC5082789

Schlebach JP, Barrett PJ, Day CA, Kim JH, Kenworthy AK, Sanders CR, Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles., 2016; Biochemistry. 55(7) 985-8 PMID: 26859249 | PMCID: PMC4766968

Chandrasekaran R, Kenworthy AK, Lacy DB, Clostridium difficile Toxin A Undergoes Clathrin-Independent, PACSIN2-Dependent Endocytosis., 2016; PLoS pathogens. 12(12) e1006070 PMID: 27942025 | PMCID: PMC5152916

Kraft LJ, Manral P, Dowler J, Kenworthy AK, Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus., 2016; Traffic (Copenhagen, Denmark). 17(4) 369-99 PMID: 26728248 | PMCID: PMC4975375

Raghunathan K, Wong TH, Chinnapen DJ, Lencer WI, Jobling MG, Kenworthy AK, Glycolipid Crosslinking Is Required for Cholera Toxin to Partition Into and Stabilize Ordered Domains., 2016; Biophysical journal. 111(12) 2547-2550 PMID: 27914621 | PMCID: PMC5192692

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S..., Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)., 2016; Autophagy. 12(1) 1-222 PMID: 26799652 | PMCID: PMC4835977

Kang M, Andreani M, Kenworthy AK, Validation of Normalizations, Scaling, and Photofading Corrections for FRAP Data Analysis., 2015; PloS one. 10(5) e0127966 PMID: 26017223 | PMCID: PMC4446327

Kenworthy AK, Lipid Domains. Preface., 2015; Current topics in membranes. 75() xiii-xvii PMID: 26015288 | PMCID: PMC4984669

Day CA, Baetz NW, Copeland CA, Kraft LJ, Han B, Tiwari A, Drake KR, De Luca H, Chinnapen DJ, Davidson MW, Holmes RK, Jobling MG, Schroer TA, Lencer WI, Kenworthy AK, Microtubule motors power plasma membrane tubulation in clathrin-independent endocytosis., 2015; Traffic (Copenhagen, Denmark). 16(6) 572-90 PMID: 25690058 | PMCID: PMC4440230

Day CA, Kenworthy AK, Functions of cholera toxin B-subunit as a raft cross-linker., 2015; Essays in biochemistry. 57() 135-45 PMID: 25658350 | PMCID: PMC4346142

Han B, Tiwari A, Kenworthy AK, Tagging strategies strongly affect the fate of overexpressed caveolin-1., 2015; Traffic (Copenhagen, Denmark). 16(4) 417-38 PMID: 25639341 | PMCID: PMC4440517

Prewitt AR, Ghose S, Frump AL, Datta A, Austin ED, Kenworthy AK, de Caestecker MP, Heterozygous null bone morphogenetic protein receptor type 2 mutations promote SRC kinase-dependent caveolar trafficking defects and endothelial dysfunction in pulmonary arterial hypertension., 2014; The Journal of biological chemistry. 290(2) 960-71 PMID: 25411245 | PMCID: PMC4294523

Kraft LJ, Nguyen TA, Vogel SS, Kenworthy AK, Size, stoichiometry, and organization of soluble LC3-associated complexes., 2014; Autophagy. 10(5) 861-77 PMID: 24646892 | PMCID: PMC4768459

Mazerik JN, Kraft LJ, Kenworthy AK, Tyska MJ, Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics., 2014; Biophysical journal. 106(3) 649-58 PMID: 24507605 | PMCID: PMC3944834

Renard HF, Simunovic M, Lemi?re J, Boucrot E, Garcia-Castillo MD, Arumugam S, Chambon V, Lamaze C, Wunder C, Kenworthy AK, Schmidt AA, McMahon HT, Sykes C, Bassereau P, Johannes L, Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis., 2014; Nature. 517(7535) 493-6 PMID: 25517096 | PMCID: PMC4342003

Song Y, Kenworthy AK, Sanders CR, Cholesterol as a co-solvent and a ligand for membrane proteins., 2013; Protein science : a publication of the Protein Society. 23(1) 1-22 PMID: 24155031 | PMCID: PMC3892294

Hanson CA, Drake KR, Baird MA, Han B, Kraft LJ, Davidson MW, Kenworthy AK, Overexpression of caveolin-1 is sufficient to phenocopy the behavior of a disease-associated mutant., 2013; Traffic (Copenhagen, Denmark). 14(6) 663-77 PMID: 23469926 | PMCID: PMC3674505

Kraft LJ, Kenworthy AK, Imaging protein complex formation in the autophagy pathway: analysis of the interaction of LC3 and Atg4B(C74A) in live cells using F?rster resonance energy transfer and fluorescence recovery after photobleaching., 2012; Journal of biomedical optics. 17(1) 011008 PMID: 22352642 | PMCID: PMC3380812

Day CA, Kenworthy AK, Mechanisms underlying the confined diffusion of cholera toxin B-subunit in intact cell membranes., 2012; PloS one. 7(4) e34923 PMID: 22511973 | PMCID: PMC3325267

Chinnapen DJ, Hsieh WT, te Welscher YM, Saslowsky DE, Kaoutzani L, Brandsma E, D'Auria L, Park H, Wagner JS, Drake KR, Kang M, Benjamin T, Ullman MD, Costello CE, Kenworthy AK, Baumgart T, Massol RH, Lencer WI, Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1., 2012; Developmental cell. 23(3) 573-86 PMID: 22975326 | PMCID: PMC3443397

Kang M, Day CA, Kenworthy AK, DiBenedetto E, Simplified equation to extract diffusion coefficients from confocal FRAP data., 2012; Traffic (Copenhagen, Denmark). 13(12) 1589-600 PMID: 22984916 | PMCID: PMC3731631

Day CA, Kraft LJ, Kang M, Kenworthy AK, Analysis of protein and lipid dynamics using confocal fluorescence recovery after photobleaching (FRAP)., 2012; Current protocols in cytometry. 0() Unit2.19 PMID: 23042527 | PMCID: PMC3538152

Kang M, DiBenedetto E, Kenworthy AK, Proposed correction to Feder's anomalous diffusion FRAP equations., 2011; Biophysical journal. 100(3) 791-792 PMID: 21281595 | PMCID: PMC3030171

Lapierre LA, Ducharme NA, Drake KR, Goldenring JR, Kenworthy AK, Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells., 2011; Experimental cell research. 318(2) 103-13 PMID: 22036648 | PMCID: PMC3230713